Microbiology

Items 18

Microbiology

Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study

Sara Mohammed Mayas, Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Aref Noman

2390-2397

2026-07-28

DOI: 10.59628/jast.v4i7.2963

Abstract

Background: Helicobacter pylori infection remains a major global public health challenge, particularly in low-resource settings where disease burden remains high. Despite its clinical importance, data regarding associated risk factors and symptom patterns remain limited in several populations, including Yemen.
Objective: This study aimed to investigate the prevalence of H. pylori infection and evaluate associated factors and clinical symptoms among gastrointestinal patients attending a tertiary healthcare center in Dhamar.
Methods: A hospital-based cross-sectional study was conducted using a quota-based sampling approach. H. pylori infection was diagnosed using a stool antigen test. Data were analyzed using univariate statistical analyses followed by multivariable binary logistic regression to identify independent predictors while controlling for potential confounding variables.
Results: The study demonstrated a high prevalence of H. pylori infection among the investigated population. Although univariate analysis initially identified several significant sociodemographic associations, multivariable logistic regression revealed that age >30 years was the only statistically significant independent predictor associated with infection (AOR = 0.086; 95% CI: 0.017–0.437; P = 0.003). Clinical manifestations, including upper abdominal pain (98.0%), nausea (97.6%), and vomiting (96.9%), showed significant associations with H. pylori positivity (P < 0.001). In contrast, lifestyle and dietary variables, including hot pepper consumption, did not retain significance following multivariable adjustment
Conclusions: H. pylori infection was highly prevalent and independently associated with older age and gastrointestinal symptoms. These findings provide preliminary epidemiological evidence and support the need for future longitudinal studies using representative sampling and confirmatory diagnostic methods.

Keywords

prevalence associated factors gastrointestinal symptoms epidemiology stool antigen test Yemen cross-sectional study.
How to Cite This Article
Sara Mohammed Mayas, Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Aref Noman (2026). Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study. Sana'a University Journal of Applied Sciences and Technology, 2390-2397. DOI: https://doi.org/10.59628/jast.v4i7.2963

Sara Mohammed Mayas, Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Aref Noman . “Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study.” Sana'a University Journal of Applied Sciences and Technology, 2026, pp. 2390-2397. https://doi.org/10.59628/jast.v4i7.2963. Accessed 20 Sep. 2026.

Sara Mohammed Mayas, Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Aref Noman . 2026. “Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study.” Sana'a University Journal of Applied Sciences and Technology : 2390-2397. DOI: https://doi.org/10.59628/jast.v4i7.2963

Sara Mohammed Mayas, Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Aref Noman (2026) ‘Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study’, Sana'a University Journal of Applied Sciences and Technology, pp. 2390-2397. doi: 10.59628/jast.v4i7.2963

@article{jast2026_art2963, author = {Sara Mohammed Mayas, Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Aref Noman }, title = {Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2026}, month = {July}, pages = {2390-2397}, doi = {10.59628/jast.v4i7.2963}, url = {https://doi.org/10.59628/jast.v4i7.2963},}

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Determinants and Clinical Manifestations of Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar, Yemen: A Hospital-Based Cross-Sectional Study

Sara Mohammed Mayas Corresponding s.mayas@su.edu.ye

Salwa H. Alkhyat s.alkhyat@su.edu.ye

Maged Ahmed Al-Garadi magedalj@gmail.com

Aref Noman arefalafif@gmail.com

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A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025)

Mohammed N. Q. Al-Bana, Saeed M. S. Al-Ghalibi, Qais Y. M. Abdullah, Saad Al-Arnoot, Assem Al-Thobhani, Basim Al-Akhali

891-897

2025-06-30

DOI: 10.59628/jast.v3i3.1672

Abstract

Campylobacter jejuni is a leading bacterial agent of foodborne illnesses worldwide, with poultry serving as its
primary reservoir. This systematic review aimed to document and compare the prevalence of C. jejuni in chickens across countries in the MENA region from 2014 to early 2025. A structured search of the PubMed, Scopus, and Google Scholar databases was conducted to identify peer-reviewed studies that reported prevalence data. Twenty-eight studies from 13 MENA countries met the inclusion criteria, covering a range of sample types and diagnostic methods, including culture and PCR. The reported prevalence varies widely, from as low as 6% to over 80%, largely influenced by sampling methods, biosecurity practices, and environmental conditions. Algeria and Iran are among the countries with the highest reported prevalence rates. A meta-analysis was not feasible owing to methodological heterogeneity. Therefore, the findings were synthesized descriptively. The results emphasize significant gaps in poultry hygiene practices and surveillance infrastructure, which may contribute to the ongoing public health burden of C. jejuni in the region. Additionally, this review highlights emerging antimicrobial resistance trends associated with poultry farming. These findings underscore the urgency of coordinated regional efforts to enhance epidemiological monitoring and implement evidence-based poultry safety measures.

Keywords

Campylobacter jejuni, Prevalence Middle East Poultry Food safety
How to Cite This Article
Mohammed N. Q. Al-Bana, Saeed M. S. Al-Ghalibi, Qais Y. M. Abdullah, Saad Al-Arnoot, Assem Al-Thobhani, Basim Al-Akhali (2025). A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025). Sana'a University Journal of Applied Sciences and Technology, 891-897. DOI: https://doi.org/10.59628/jast.v3i3.1672

Mohammed N. Q. Al-Bana, Saeed M. S. Al-Ghalibi, Qais Y. M. Abdullah, Saad Al-Arnoot, Assem Al-Thobhani, Basim Al-Akhali . “A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025).” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 891-897. https://doi.org/10.59628/jast.v3i3.1672. Accessed 20 Sep. 2026.

Mohammed N. Q. Al-Bana, Saeed M. S. Al-Ghalibi, Qais Y. M. Abdullah, Saad Al-Arnoot, Assem Al-Thobhani, Basim Al-Akhali . 2025. “A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025).” Sana'a University Journal of Applied Sciences and Technology : 891-897. DOI: https://doi.org/10.59628/jast.v3i3.1672

Mohammed N. Q. Al-Bana, Saeed M. S. Al-Ghalibi, Qais Y. M. Abdullah, Saad Al-Arnoot, Assem Al-Thobhani, Basim Al-Akhali (2025) ‘A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025)’, Sana'a University Journal of Applied Sciences and Technology, pp. 891-897. doi: 10.59628/jast.v3i3.1672

@article{jast2025_art1672, author = {Mohammed N. Q. Al-Bana, Saeed M. S. Al-Ghalibi, Qais Y. M. Abdullah, Saad Al-Arnoot, Assem Al-Thobhani, Basim Al-Akhali }, title = {A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025)}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {June}, pages = {891-897}, doi = {10.59628/jast.v3i3.1672}, url = {https://doi.org/10.59628/jast.v3i3.1672},}

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A systematic review of the prevalence of Campylobacter jejuni in chicken populations within Middle East and North Africa (2014–2025)

Mohammed N. Q. Al-Bana Corresponding m.albana@su.edu.ye

Saeed M. S. Al-Ghalibi m.albana@su.edu.ye

Qais Y. M. Abdullah m.albana@su.edu.ye

Saad Al-Arnoot m.albana@su.edu.ye

Assem Al-Thobhani m.albana@su.edu.ye

Basim Al-Akhali m.albana@su.edu.ye

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Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds

Miadah M. Mojahed, Abdulrahman A. Humaid

1545-1551

2026-01-29

DOI: 10.59628/jast.v4i1.1931

Abstract

Antimicrobial agents encompass a wide range of compounds, including antibiotics, bacteriocins, and lipopeptides, which play a crucial role in combating infectious diseases. Antibiotics, in particular, are secondary metabolites of low molecular weight predominantly synthesized by soil dwelling microorganisms. These microbial metabolites have long served as a vital source of clinically important therapeutic agents. Members of the genus Bacillus and other rhizosphere-associated bacteria are especially known for producing diverse antimicrobial substances. In this study, ten rhizosphere soil samples were collected from different sites in Sana’a city, Yemen. From these samples, 50 antibiotic-producing soil bacteria were isolated. Bioactive metabolites were extracted using the solvent extraction method with chloroform and Ethanol. The crude extracts were analyzed by Gas Chromatography–Mass Spectrometry (GC–MS) to identify their organic composition. Among the 50 bacterial isolates, 14 showed antibacterial activity against resistant Escherichia coli using the agar well diffusion method. Further secondary screening revealed that the filtrates of four isolates Pseudomonas fluorescens, Bacillus subtilis, Acinetobacter baylyi, and Azotobacter vinelandii exhibited the strongest antibacterial effects. GC–MS analysis showed that each isolate produced more than eighty organic compounds; however, only a subset demonstrated antibacterial activity. The most notable bioactive compounds detected included Phenol, 4-(2-aminoethyl)- (CAS: tyramine), 2,4-di-tert-butylphenol, and n-hexadecanoic acid. This study highlights the potential of rhizosphere soil bacteria as promising sources of novel bioactive compounds. The identification of active metabolites and their antibacterial properties against resistant E. coli underscores their possible application in the development of alternative therapeutic strategiesto address antibiotic resistance.

Keywords

Secondary metabolites GC-MS spectrophotometer Escherichia coli Soil bacteria Organic compounds Chloroform Ethanol
How to Cite This Article
Miadah M. Mojahed, Abdulrahman A. Humaid (2026). Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds. Sana'a University Journal of Applied Sciences and Technology, 1545-1551. DOI: https://doi.org/10.59628/jast.v4i1.1931

Miadah M. Mojahed, Abdulrahman A. Humaid . “Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds.” Sana'a University Journal of Applied Sciences and Technology, 2026, pp. 1545-1551. https://doi.org/10.59628/jast.v4i1.1931. Accessed 20 Sep. 2026.

Miadah M. Mojahed, Abdulrahman A. Humaid . 2026. “Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds.” Sana'a University Journal of Applied Sciences and Technology : 1545-1551. DOI: https://doi.org/10.59628/jast.v4i1.1931

Miadah M. Mojahed, Abdulrahman A. Humaid (2026) ‘Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds’, Sana'a University Journal of Applied Sciences and Technology, pp. 1545-1551. doi: 10.59628/jast.v4i1.1931

@article{jast2026_art1931, author = {Miadah M. Mojahed, Abdulrahman A. Humaid }, title = {Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2026}, month = {January}, pages = {1545-1551}, doi = {10.59628/jast.v4i1.1931}, url = {https://doi.org/10.59628/jast.v4i1.1931},}

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Antibacterial Activity of Soil Bacteria against Escherichia coli and GC-MS Analysis of their Organic Compounds

Miadah M. Mojahed Corresponding miadahmojahed98@gmail.com

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Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens

Nesrain A. Al-Mekhlafi, Kholoud G. Badi, Roua A. Al-Refai, Sara M. Al-bana’a

1520-1527

2026-01-29

DOI: 10.59628/jast.v4i1.2137

Abstract

Bacillus species represent one of the most dominant soil bacteria and are capable of producing diverse metabolites with antimicrobial properties. This study aimed to isolate Bacillus spp. from Yemeni soils and evaluate their antimicrobial activity. Thirty soil samples were collected, and the isolates were characterized using phenotypic and biochemical tests. Their antimicrobial potential was assessed against Staphylococcus aureus, Escherichia coli, Pseudomonas sp., Salmonella sp., and Candida albicans using agar disc and well diffusion methods. In agar disc assays, the strongest inhibitory effects were observed against Salmonella sp. (13–32.5 mm), followed by E. coli (13–25.4 mm), C. albicans (10–18.5 mm), and S. aureus (10–15.5 mm). Five isolates exhibited antibacterial activity against Salmonella sp. in well diffusion assay. These results highlight the significant antimicrobial potential of Bacillus isolates derived from Yemeni soils. Further studies are recommended to identify bioactive metabolites and explore their suitability for the development of novel therapeutic agents.

Keywords

Soil <em> Bacillus</em> spp. Antimicrobial activity Human pathogens.
How to Cite This Article
Nesrain A. Al-Mekhlafi, Kholoud G. Badi, Roua A. Al-Refai, Sara M. Al-bana’a (2026). Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens. Sana'a University Journal of Applied Sciences and Technology, 1520-1527. DOI: https://doi.org/10.59628/jast.v4i1.2137

Nesrain A. Al-Mekhlafi, Kholoud G. Badi, Roua A. Al-Refai, Sara M. Al-bana’a . “Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens.” Sana'a University Journal of Applied Sciences and Technology, 2026, pp. 1520-1527. https://doi.org/10.59628/jast.v4i1.2137. Accessed 20 Sep. 2026.

Nesrain A. Al-Mekhlafi, Kholoud G. Badi, Roua A. Al-Refai, Sara M. Al-bana’a . 2026. “Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens.” Sana'a University Journal of Applied Sciences and Technology : 1520-1527. DOI: https://doi.org/10.59628/jast.v4i1.2137

Nesrain A. Al-Mekhlafi, Kholoud G. Badi, Roua A. Al-Refai, Sara M. Al-bana’a (2026) ‘Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens’, Sana'a University Journal of Applied Sciences and Technology, pp. 1520-1527. doi: 10.59628/jast.v4i1.2137

@article{jast2026_art2137, author = {Nesrain A. Al-Mekhlafi, Kholoud G. Badi, Roua A. Al-Refai, Sara M. Al-bana’a }, title = {Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2026}, month = {January}, pages = {1520-1527}, doi = {10.59628/jast.v4i1.2137}, url = {https://doi.org/10.59628/jast.v4i1.2137},}

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Antimicrobial Antimicrobial activity of Bacillus species isolated from Yemeni soils against some human pathogens

Nesrain A. Al-Mekhlafi Corresponding n.almikhlafi@su.edu.com

Kholoud G. Badi khlooud7783@gmail.com

Roua A. Al-Refai rouaalrefai2@mail.com

Sara M. Al-bana’a sartalbna59@gmail.com

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Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator

Basem Al-Akhali, Qais Y. M. Abdullah, Maysoon A. Al-Zubairy, Mohammed N. Q. Al-Bana, Assem Al-Thobhani, Saad Al-Arnoot

1195-1202

2025-10-28

DOI: 10.59628/jast.v3i5.2044

Abstract

Meat products are very perishable foodstuff that can easily be contaminated by microbial organisms and spoiled due poor storage and handling facilities. The current research was conducted to determine microbiological quality of imported meat products in Yemeni retail market with emphasis on use of Pseudomonas spp. to test the level of spoilage. A total of 214 samples that included beef burgers, chicken burgers, chicken meat, minced beef and minced lamb were aseptically collected at the 2 supermarkets in Sanaa in Yemen. Each sample was analyzed by standard culture-based procedures to achieve total aerobic plate count (APC), and presence of Pseudomonas spp., followed by species-level identification with the help of VITEK(R) 2 Compact system. The number of minced beef samples and minced lamb samples had the highest mean APC values of 8.87 10 5 and 8.55 10 5 trend, respectively, lower values were found in burger samples and chicken meat samples. The percentage rates of detection of Pseudomonas spp. were 16.36 with the maximum percentages found in minced lamb (35.29) and minced beef (25.00). Pseudomonas aeruginosa (60ATTR Morphological identification of Pseudomonas spp. showed that P. aeruginosa was the commonest isolate (60%) followed by P. fluorescens (40%). The samples were all compliant with the Yemeni GSO 1016:2019 and 83.64 percent with the ISO 13720:2010, which states that the microorganisms must not have Pseudomonas spp. Such results support the increased susceptibility of minced meats towards microbial contamination and putrifaction. In revising this study, the researchers suggest that they put Pseudomonas spp. monitoring as part of the national food safety policy and strengthening the cold chain to increase meat quality control and safeguard the health of the people in Yemen.

Keywords

Meat products Pseudomonas spp Microbiological quality Spoilage indicator Yemen
How to Cite This Article
Basem Al-Akhali, Qais Y. M. Abdullah, Maysoon A. Al-Zubairy, Mohammed N. Q. Al-Bana, Assem Al-Thobhani, Saad Al-Arnoot (2025). Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator. Sana'a University Journal of Applied Sciences and Technology, 1195-1202. DOI: https://doi.org/10.59628/jast.v3i5.2044

Basem Al-Akhali, Qais Y. M. Abdullah, Maysoon A. Al-Zubairy, Mohammed N. Q. Al-Bana, Assem Al-Thobhani, Saad Al-Arnoot . “Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1195-1202. https://doi.org/10.59628/jast.v3i5.2044. Accessed 20 Sep. 2026.

Basem Al-Akhali, Qais Y. M. Abdullah, Maysoon A. Al-Zubairy, Mohammed N. Q. Al-Bana, Assem Al-Thobhani, Saad Al-Arnoot . 2025. “Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator.” Sana'a University Journal of Applied Sciences and Technology : 1195-1202. DOI: https://doi.org/10.59628/jast.v3i5.2044

Basem Al-Akhali, Qais Y. M. Abdullah, Maysoon A. Al-Zubairy, Mohammed N. Q. Al-Bana, Assem Al-Thobhani, Saad Al-Arnoot (2025) ‘Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator’, Sana'a University Journal of Applied Sciences and Technology, pp. 1195-1202. doi: 10.59628/jast.v3i5.2044

@article{jast2025_art2044, author = {Basem Al-Akhali, Qais Y. M. Abdullah, Maysoon A. Al-Zubairy, Mohammed N. Q. Al-Bana, Assem Al-Thobhani, Saad Al-Arnoot }, title = {Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {October}, pages = {1195-1202}, doi = {10.59628/jast.v3i5.2044}, url = {https://doi.org/10.59628/jast.v3i5.2044},}

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Assessment of Microbiological Quality of Imported Meat Products in Yemeni markets with Focus on Pseudomonas spp. as a Spoilage Indicator

Basem Al-Akhali Corresponding b.alakhali@su.edu.ye

Qais Y. M. Abdullah b.alakhali@su.edu.ye

Maysoon A. Al-Zubairy b.alakhali@su.edu.ye

Mohammed N. Q. Al-Bana b.alakhali@su.edu.ye

Assem Al-Thobhani b.alakhali@su.edu.ye

Saad Al-Arnoot b.alakhali@su.edu.ye

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Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics

Abdulrahman A. Humaid, Maher Ali Al-maqtari, Abdulkarim Kassem Alzomor, Anes A.M. Thabit

168-174

2024-05-05

DOI: 10.59628/jast.v2i2.838

Abstract

The spread of carbapenem-resistant Klebsiella pneumoniae (CRKp), which is one of the top pathogens in the WHO list of antibiotic-resistant bacteria, is a true threat to human life. This study aimed to determine the prevalence, carbapenemase phenotypes, and resistance to last-resort antibiotics of CRKp isolated from clinical specimens of inpatients and outpatients in 3 health-service facilities in Sana‘a-Yemen between December 2020 and November 2022. The carbapenemases produced by these isolates were phenotyped by mCIM and eCIM, and the susceptibility of the isolates to last-resort antibiotics was determined using the disk diffusion method. A total of 140 CRKp isolates were recovered in this study, mostly from urine specimens (35 %), from inpatients hospitalized in the gynecology ward (30%) and intensive care units (22.9 %). Differences in CRKp prevalence were insignificant between females and males and between age ranges but significant between urban and rural residents. Moreover, 75.7% of CRKp isolates were producers of metallo-β-lactamases and 81.4, 78.6 %, 17.1%, 15.7 %, and 7.1% of these isolates were resistant to aztreonam, ceftazidime+ avibactam, colistin, fosfomycin, and tigecycline, respectively. In conclusion, there is a considerable prevalence of CRKp isolates among Yemeni patients. These isolates are mostly metallo β-lactamase producers and have remarkable resistance to most lastresort antibiotics. Accordingly, there is a need to establish and employ urgent control and prevention measures to diminish the spread of CRKp and limit its emergent resistance to last-resort antibiotics in our community.

Keywords

Carbapenem-Resistant Klebsiella Pneumoniae Prevalence Yemen Metallo-β-lactamases Last-Resort Antibiotics
How to Cite This Article
Abdulrahman A. Humaid, Maher Ali Al-maqtari, Abdulkarim Kassem Alzomor, Anes A.M. Thabit (2024). Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics. Sana'a University Journal of Applied Sciences and Technology, 168-174. DOI: https://doi.org/10.59628/jast.v2i2.838

Abdulrahman A. Humaid, Maher Ali Al-maqtari, Abdulkarim Kassem Alzomor, Anes A.M. Thabit . “Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics.” Sana'a University Journal of Applied Sciences and Technology, 2024, pp. 168-174. https://doi.org/10.59628/jast.v2i2.838. Accessed 20 Sep. 2026.

Abdulrahman A. Humaid, Maher Ali Al-maqtari, Abdulkarim Kassem Alzomor, Anes A.M. Thabit . 2024. “Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics.” Sana'a University Journal of Applied Sciences and Technology : 168-174. DOI: https://doi.org/10.59628/jast.v2i2.838

Abdulrahman A. Humaid, Maher Ali Al-maqtari, Abdulkarim Kassem Alzomor, Anes A.M. Thabit (2024) ‘Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics’, Sana'a University Journal of Applied Sciences and Technology, pp. 168-174. doi: 10.59628/jast.v2i2.838

@article{jast2024_art838, author = {Abdulrahman A. Humaid, Maher Ali Al-maqtari, Abdulkarim Kassem Alzomor, Anes A.M. Thabit }, title = {Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2024}, month = {May}, pages = {168-174}, doi = {10.59628/jast.v2i2.838}, url = {https://doi.org/10.59628/jast.v2i2.838},}

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Carbapenem-Resistant Klebsiella pneumoniae in Yemeni Patients: Prevalence, Phenotypes, and Resistance Profile to Last-Resort Antibiotics

Abdulrahman A. Humaid Corresponding a-hamid@su.edu.ye

Maher Ali Al-maqtari m.almaqtari@su.edu.ye

Abdulkarim Kassem Alzomor alzomor@tu.edu.ye

Anes A.M. Thabit aneesalabsi1973@gmail.com

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Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen

Abdulraqeb Ali Alshami, Abdu-Alraoof Al-shawkany, Saleh A. M. A. ALomaisi, Hamid Ali Alrefaiey, Bashar Saleh Al-Mawti

1299-1305

2025-12-28

DOI: 10.59628/jast.v3i6.2185

Abstract

The present study aimed to investigate the clinical and haemato biochemical alterations in sheep and goats infected with gastrointestinal helminths in Sana’a Governorate, Yemen. A total of 140 adult local sheep (n=79) and goats (n=61) were examined during the period from August to December 2024, of which 59 sheep and 41 goats were identified as helminth-infected based on clinical signs and faecal examination, while 20 animals of each species served as controls (non-infected). Blood samples were collected for the analysis of haematological and biochemical parameters. Clinical examination revealed anemia, diarrhea, emaciation, pale mucous membranes, mild fever, and increased pulse rate in infected animals. Parasitological analysis confirmed infections with a range of nematodes, trematodes and cestodes including Ostertagia, Haemonchus, Trichuris, Nematodirus, Fasciola, and Moniezia. Haematological findings showed significantly reduced Hb, PCV, and TEC, with elevated eosinophil counts but no significant changes in ESR, TLC, or other leukocytes. Biochemical analysis demonstrated significant decreases in total protein, albumin, and glucose, accompanied by elevated AST and ALT, while globulin levels remained unchanged. The study concluded that sheep and goats suffering from gastrointestinal helminths showed altered status of clinical, haematological and biochemical parameters. Effective control measures should be managed to prevent those harmful effects of gastrointestinal helminths on small ruminants.

Keywords

Clinical Haemato-biochemical Gastrointestinal helminths Sheep Goats
How to Cite This Article
Abdulraqeb Ali Alshami, Abdu-Alraoof Al-shawkany, Saleh A. M. A. ALomaisi, Hamid Ali Alrefaiey, Bashar Saleh Al-Mawti (2025). Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen. Sana'a University Journal of Applied Sciences and Technology, 1299-1305. DOI: https://doi.org/10.59628/jast.v3i6.2185

Abdulraqeb Ali Alshami, Abdu-Alraoof Al-shawkany, Saleh A. M. A. ALomaisi, Hamid Ali Alrefaiey, Bashar Saleh Al-Mawti . “Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1299-1305. https://doi.org/10.59628/jast.v3i6.2185. Accessed 20 Sep. 2026.

Abdulraqeb Ali Alshami, Abdu-Alraoof Al-shawkany, Saleh A. M. A. ALomaisi, Hamid Ali Alrefaiey, Bashar Saleh Al-Mawti . 2025. “Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 1299-1305. DOI: https://doi.org/10.59628/jast.v3i6.2185

Abdulraqeb Ali Alshami, Abdu-Alraoof Al-shawkany, Saleh A. M. A. ALomaisi, Hamid Ali Alrefaiey, Bashar Saleh Al-Mawti (2025) ‘Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 1299-1305. doi: 10.59628/jast.v3i6.2185

@article{jast2025_art2185, author = {Abdulraqeb Ali Alshami, Abdu-Alraoof Al-shawkany, Saleh A. M. A. ALomaisi, Hamid Ali Alrefaiey, Bashar Saleh Al-Mawti }, title = {Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {December}, pages = {1299-1305}, doi = {10.59628/jast.v3i6.2185}, url = {https://doi.org/10.59628/jast.v3i6.2185},}

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Cross-Sectional Study on Clinical and Haemato-Biochemical Profile in Sheep and Goats Infected with Gastrointestinal Helminths in Sana'a Governorate, Yemen

Abdulraqeb Ali Alshami Corresponding a.shami@su.edu.ye

Abdu-Alraoof Al-shawkany a.shami@su.edu.ye

Saleh A. M. A. ALomaisi a.shami@su.edu.ye

Hamid Ali Alrefaiey a.shami@su.edu.ye

Bashar Saleh Al-Mawti a.shami@su.edu.ye

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Effect of heavy metals on the growth of Bacillus spp. isolates -

Maysoon Al Zubairy, Lara S. Alburihi

514-519

2024-12-31

DOI: 10.59628/jast.v2i6.1310

Abstract

The objective of this study was to investigate the effects of different concentrations of heavy metals,: namely Cobalt (Co), Cadmium (Cd), manganese (Mn), Copper (Cu), Mercury (Hg), Iron (F)e, Zinc (Zn), and Lead (Pb), on four different species of Bacillus (Bacillus subitils sub sp. subitilus, B. atrophaeus, B. mycoides, B. weihenstephananensis). Various metal concentrations (75, 125, 250, 500, and 750 µg ml^-1) of each heavy metal salt were prepared and tested against the target organisms. This study showed that Bacillus spp. exhibited different responses to the different concentrations of heavy metals. Among the tested strains, B. atrophaeus and B. subtilils sub sp. subitils were the most sensitive to varying concentrations of the studied heavy metals. Cd was the most effective heavy metal on all tested strains, whereas all isolates were resistant to all concentrations of Mn and Pb. Most of the strains were resistant to different concentrations of Cu and Fe.

Keywords

Heavy metals Growth Effect Bacillus spp Tolerance Lead Mercury
How to Cite This Article
Maysoon Al Zubairy, Lara S. Alburihi (2024). Effect of heavy metals on the growth of Bacillus spp. isolates. Sana'a University Journal of Applied Sciences and Technology, 514-519. DOI: https://doi.org/10.59628/jast.v2i6.1310

Maysoon Al Zubairy, Lara S. Alburihi . “Effect of heavy metals on the growth of Bacillus spp. isolates.” Sana'a University Journal of Applied Sciences and Technology, 2024, pp. 514-519. https://doi.org/10.59628/jast.v2i6.1310. Accessed 20 Sep. 2026.

Maysoon Al Zubairy, Lara S. Alburihi . 2024. “Effect of heavy metals on the growth of Bacillus spp. isolates.” Sana'a University Journal of Applied Sciences and Technology : 514-519. DOI: https://doi.org/10.59628/jast.v2i6.1310

Maysoon Al Zubairy, Lara S. Alburihi (2024) ‘Effect of heavy metals on the growth of Bacillus spp. isolates’, Sana'a University Journal of Applied Sciences and Technology, pp. 514-519. doi: 10.59628/jast.v2i6.1310

@article{jast2024_art1310, author = {Maysoon Al Zubairy, Lara S. Alburihi }, title = {Effect of heavy metals on the growth of Bacillus spp. isolates}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2024}, month = {December}, pages = {514-519}, doi = {10.59628/jast.v2i6.1310}, url = {https://doi.org/10.59628/jast.v2i6.1310},}

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Effect of heavy metals on the growth of Bacillus spp. isolates

Maysoon Al Zubairy Corresponding m.alzubairy@su.edu.ye

Lara S. Alburihi alburihilara@gmail.com

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Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen

Ghadeer Al Obahi, Saeed M. Al Ghalibi, Hala J. Al Jobory

1291-1298

2025-12-28

DOI: 10.59628/jast.v3i6.2004

Abstract

Background: The surfaces of medical laboratories are potential reservoirs of multidrug-resistant (MDR) organisms, posing a significant biosafety risk. In Yemen, where national surveillance and laboratory biosafety data are limited, the extent of environmental contamination is unknown. This study aimed to provide the first characterization of bacterial flora, their antimicrobial resistance profiles, and virulence-associated enzyme production from isolates on laboratory surfaces in Sana’a.
Methods: In this descriptive microbiological study, 100 surface swabs were collected from ten medical laboratories. Bacterial identification was performed using standard biochemical methods. Antimicrobial susceptibility was determined by disk diffusion according to the EUCAST guidelines. The isolates were also screened for protease, lipase, and hemolysin production.
Results: Bacterial growth was detected in 94% (94/100) of the sampled surfaces, with the bioburden dominated by gram-positive bacteria (98.3%), primarily Staphylococcus and Bacillus spp.. The isolates exhibited high resistance rates, including near-universal resistance to ampicillin (100%) and high resistance to clindamycin (>86%) among staphylococci. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in 15.6% of S.aureus isolates. Many isolates, particularly Bacillus species, exhibit significant enzymatic activity.
Conclusion: Surfaces in medical laboratories in Sana’a harbor a significant bioburden of pathogenic and MDR bacteria, including MRSA, along with isolates with notable enzymatic capabilities. These baseline findings provide crucial data-driven evidence to inform local biosafety policies and highlight the urgent need to integrate environmental surveillance into Yemen’s national AMR action plan.

Keywords

Bacterial Contamination Medical Laboratories Antibiotic Resistance MRSA Multidrug Resistance Staphylococcus Bacillus Environmental Surveillance Yemen
How to Cite This Article
Ghadeer Al Obahi, Saeed M. Al Ghalibi, Hala J. Al Jobory (2025). Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen. Sana'a University Journal of Applied Sciences and Technology, 1291-1298. DOI: https://doi.org/10.59628/jast.v3i6.2004

Ghadeer Al Obahi, Saeed M. Al Ghalibi, Hala J. Al Jobory . “Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1291-1298. https://doi.org/10.59628/jast.v3i6.2004. Accessed 20 Sep. 2026.

Ghadeer Al Obahi, Saeed M. Al Ghalibi, Hala J. Al Jobory . 2025. “Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 1291-1298. DOI: https://doi.org/10.59628/jast.v3i6.2004

Ghadeer Al Obahi, Saeed M. Al Ghalibi, Hala J. Al Jobory (2025) ‘Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 1291-1298. doi: 10.59628/jast.v3i6.2004

@article{jast2025_art2004, author = {Ghadeer Al Obahi, Saeed M. Al Ghalibi, Hala J. Al Jobory }, title = {Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {December}, pages = {1291-1298}, doi = {10.59628/jast.v3i6.2004}, url = {https://doi.org/10.59628/jast.v3i6.2004},}

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Enzymatic Capabilities and Antibiotic Susceptibility of Bacteria Isolated from Medical Laboratory Surfaces in Sana’a, Yemen

Ghadeer Al Obahi Corresponding alobahighmicrobiology@gmail.com

Saeed M. Al Ghalibi s.alghalabi@su.edu.ye

Hala J. Al Jobory h.aljubouri@su.edu.ye

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Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen

Hassan Ibrahim, Ghassan H. Al-madani, Q. Y. M. Abdullah, H. A. Al-Shamahy

232-240

2024-06-29

DOI: 10.59628/jast.v2i3.978

Abstract

Oral flora is important for maintaining dental health, but some situations may disturb the oral microbiota, which can support the growth of Microbial Pathogens. 28 pathogenic bacteria:17 G+ve and 11 G-ve) were isolated from dental plaques of 192 dental patients and identified by the VITEK 2 Compact System after culturing and studying their morphological features using Gram stain. About 13 (9 G+vecocci and 4 G-ve bacilli) and 24 (13 G+ve cocci, 1 G+ve bacilli, and 10 G-ve bacilli) pathogenic bacteria were isolated from the dental plaques of 46 males and 146 Female patients, respectively. Moreover, Streptococcus mitis exhibited the highest prevalence among the 28 isolated pathogenic bacteria. Furthermore, Serratia marcescens, and Streptococcus parasanguinis showed the highest mean number of colonies among the pathogenic bacteria isolated from male patients with dental plaques. Similarly, Serratia odorifera exhibited the highest mean number of colonies among the pathogenic bacteria isolated from female Patients with dental plaques.

Keywords

Dental Plaque Pathogenic Bacteria Yemeni Dental Patients VITEK 2 Compact System Gram-Positive Bacteria Gram-Negative Bacteria
How to Cite This Article
Hassan Ibrahim, Ghassan H. Al-madani, Q. Y. M. Abdullah, H. A. Al-Shamahy (2024). Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen. Sana'a University Journal of Applied Sciences and Technology, 232-240. DOI: https://doi.org/10.59628/jast.v2i3.978

Hassan Ibrahim, Ghassan H. Al-madani, Q. Y. M. Abdullah, H. A. Al-Shamahy . “Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2024, pp. 232-240. https://doi.org/10.59628/jast.v2i3.978. Accessed 20 Sep. 2026.

Hassan Ibrahim, Ghassan H. Al-madani, Q. Y. M. Abdullah, H. A. Al-Shamahy . 2024. “Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 232-240. DOI: https://doi.org/10.59628/jast.v2i3.978

Hassan Ibrahim, Ghassan H. Al-madani, Q. Y. M. Abdullah, H. A. Al-Shamahy (2024) ‘Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 232-240. doi: 10.59628/jast.v2i3.978

@article{jast2024_art978, author = {Hassan Ibrahim, Ghassan H. Al-madani, Q. Y. M. Abdullah, H. A. Al-Shamahy }, title = {Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2024}, month = {June}, pages = {232-240}, doi = {10.59628/jast.v2i3.978}, url = {https://doi.org/10.59628/jast.v2i3.978},}

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Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen

Hassan Ibrahim h.ibrahim@su.edu.ye

Ghassan H. Al-madani Corresponding ghassan.almadani@su.edu.ye

Q. Y. M. Abdullah q.yusuf@su.edu.ye

H. A. Al-Shamahy h.shmahe@su.edu.ye

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Microbiological Quality Assessment of Ice Cream Produced in Yemen

Qais Yusuf M. Abdullah, Yusuf Ali H. Al-Zuraei, Mohammed F. Al- Helali, Basem Al-Akhali, Amal A. Abu Al-rejal, Saad Al-Arnoot

749-755

2025-04-30

DOI: 10.59628/jast.v3i2.1525

Abstract

The microbiological quality of ice cream produced in Yemen was assessed to evaluate compliance with food safety standards and identify potential health risks. A total of 100 samples were collected from three Yemeni governorates (Sana’a, Ibb, and Hodeidah) and analyzed for aerobic plate count, Enterobacteriaceae, Escherichia coli, Salmonella spp., Staphylococcus aureus, mold, and yeast counts. The results showed that 61% of the samples were contaminated, failing to meet acceptable microbial quality standards. Significant disparities were observed between large and small factories, with 52% of samples from large factories meeting the standards compared to only 26% from small factories (P = 0.013). Escherichia coli contamination was detected in 28% of small factory samples, while yeast and mold contamination was significantly higher in small factories (72%) compared to large factories (36%) (P = 0.013). Packaging type also had a significant impact on microbial quality (P = 0.003), with unpackaged samples showing substantially higher contamination rates than packaged samples. No Salmonella spp. or Staphylococcus aureus was detected in any samples, reflecting effective control of these pathogens. The findings emphasize the importance of implementing stringent hygiene practices and improving microbiological monitoring, especially in small-scale production facilities, to ensure the safety and quality of ice cream in Yemen.

Keywords

Ice cream Microbiological quality Enterobacteriaceae Escherichia coli Food safety Yemen
How to Cite This Article
Qais Yusuf M. Abdullah, Yusuf Ali H. Al-Zuraei, Mohammed F. Al- Helali, Basem Al-Akhali, Amal A. Abu Al-rejal, Saad Al-Arnoot (2025). Microbiological Quality Assessment of Ice Cream Produced in Yemen. Sana'a University Journal of Applied Sciences and Technology, 749-755. DOI: https://doi.org/10.59628/jast.v3i2.1525

Qais Yusuf M. Abdullah, Yusuf Ali H. Al-Zuraei, Mohammed F. Al- Helali, Basem Al-Akhali, Amal A. Abu Al-rejal, Saad Al-Arnoot . “Microbiological Quality Assessment of Ice Cream Produced in Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 749-755. https://doi.org/10.59628/jast.v3i2.1525. Accessed 20 Sep. 2026.

Qais Yusuf M. Abdullah, Yusuf Ali H. Al-Zuraei, Mohammed F. Al- Helali, Basem Al-Akhali, Amal A. Abu Al-rejal, Saad Al-Arnoot . 2025. “Microbiological Quality Assessment of Ice Cream Produced in Yemen.” Sana'a University Journal of Applied Sciences and Technology : 749-755. DOI: https://doi.org/10.59628/jast.v3i2.1525

Qais Yusuf M. Abdullah, Yusuf Ali H. Al-Zuraei, Mohammed F. Al- Helali, Basem Al-Akhali, Amal A. Abu Al-rejal, Saad Al-Arnoot (2025) ‘Microbiological Quality Assessment of Ice Cream Produced in Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 749-755. doi: 10.59628/jast.v3i2.1525

@article{jast2025_art1525, author = {Qais Yusuf M. Abdullah, Yusuf Ali H. Al-Zuraei, Mohammed F. Al- Helali, Basem Al-Akhali, Amal A. Abu Al-rejal, Saad Al-Arnoot }, title = {Microbiological Quality Assessment of Ice Cream Produced in Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {April}, pages = {749-755}, doi = {10.59628/jast.v3i2.1525}, url = {https://doi.org/10.59628/jast.v3i2.1525},}

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Microbiological Quality Assessment of Ice Cream Produced in Yemen

Qais Yusuf M. Abdullah q.yusuf@su.edu.ye

Yusuf Ali H. Al-Zuraei yousifzy55@gmail.com

Mohammed F. Al- Helali m.alhelali@su.edu.ye

Basem Al-Akhali b.alakhali@su.edu.ye

Amal A. Abu Al-rejal amalchem2014@gmail.com

Saad Al-Arnoot Corresponding s.alarnoot@su.edu.ye

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Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen

Ashwaq Ali Hezam Qasem Al-Arosi, Anas Ahmad Al-Mahbashi

1460-1466

2025-12-28

DOI: 10.59628/jast.v3i6.2096

Abstract

Background: Urinary tract infections pose a significant public health challenge in Yemen, exacerbated by the emergence of extended spectrum -lactamase-producing uropathogenic E. coli. This study aimed to determine the prevalence and antimicrobial susceptibility profile of extended-spectrum -lactamase-producing uropathogenic E. coli in Sana’a City.
Methods: A cross-sectional study collected 359 urine samples from two major hospitals in Sana’a. Uropathogenic E. coli isolates were identified using standard methods, and ESBL production was confirmed phenotypically using the double-disk synergy test. Antimicrobial susceptibility was determined via Kirby-Bauer disk diffusion
Results: Of the samples, 155 (43.1%) were positive for uropathogenic E. coli, with 41.9% (65/155) confirmed as ESBL producers. These isolates exhibited high resistance to ampicillin, cephalosporins, and fluoroquinolones. Amikacin and meropenem remained the most effective antibiotics. Extended-spectrum -lactamase-producing uropathogenic E. coli infections showed significant associations with comorbidities including diabetes, heart disease, and urinary tract deformities (p < 0.05).
Conclusion: The high prevalence of multidrug-resistant extended spectrum -lactamase-producing uropathogenic E. coli among Yemeni urinary tract infection in Sana’a city underscores the urgent need for routine susceptibility testing and antimicrobial stewardship programs to guide treatment and preserve effective antibiotics.

Keywords

Urinary Tract Infection (UTI) Uropathogenic E. coli (UPEC) Extended-Spectrum Beta-Lactamase (ESBL) Antimicrobial Resistance (AMR) Sana'a, Yemen
How to Cite This Article
Ashwaq Ali Hezam Qasem Al-Arosi, Anas Ahmad Al-Mahbashi (2025). Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen. Sana'a University Journal of Applied Sciences and Technology, 1460-1466. DOI: https://doi.org/10.59628/jast.v3i6.2096

Ashwaq Ali Hezam Qasem Al-Arosi, Anas Ahmad Al-Mahbashi . “Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1460-1466. https://doi.org/10.59628/jast.v3i6.2096. Accessed 20 Sep. 2026.

Ashwaq Ali Hezam Qasem Al-Arosi, Anas Ahmad Al-Mahbashi . 2025. “Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 1460-1466. DOI: https://doi.org/10.59628/jast.v3i6.2096

Ashwaq Ali Hezam Qasem Al-Arosi, Anas Ahmad Al-Mahbashi (2025) ‘Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 1460-1466. doi: 10.59628/jast.v3i6.2096

@article{jast2025_art2096, author = {Ashwaq Ali Hezam Qasem Al-Arosi, Anas Ahmad Al-Mahbashi }, title = {Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {December}, pages = {1460-1466}, doi = {10.59628/jast.v3i6.2096}, url = {https://doi.org/10.59628/jast.v3i6.2096},}

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Phenotypic Detection and Antibiotic Susceptibility Pattern of Extended-Spectrum β-Lactamase-Producing Uropathogenic E. coli Isolated in Sana'a City, Yemen

Ashwaq Ali Hezam Qasem Al-Arosi Corresponding ashwaqali656@gmail.com

Anas Ahmad Al-Mahbashi anas978@hotmail.com

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Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar

Amira Al-Harethi, Qais Y. Abdullah, Hala J. Al-Jobory

103 - 106

2024-02-24

DOI: 10.59628/jast.v2i1.768

Abstract

Phytophthora infestans is a pathogen that causes late blight in crops, such as potatoes and tomatoes, posing a serious threat to the Solanaceae family. Researchers have attempted to create an affordable and effective medium for isolating and cultivating this pathogen. The aim of this study was to evaluate the efficacy of low-cost V-8 agar for mycelial growth, sporangia production, and oospore production of P. infestans and compare it with Rye A medium. To achieve this, V-8 medium was modified by replacing V-8 juice with tomato. Mycelial growth and sporulation were measured on both media after 14 days of inoculation. It was observed as a white, fluffy growth in both media. The mean radial growth was larger on the low-cost V-8 medium (76 mm) than on Rye A medium (4.4 mm). The lemon-shaped sporangia with a semipapilated structure were recorded with an average length-to-width ratio of 28.6 μm to 30.6 μm, respectively. Oospore production was greater in the low-cost V-8 medium (5x10⁴) than in Rye A medium (1x10⁴). The present study demonstrated that low-cost, locally available materials such as tomato can be used as an alternative nutrient medium for the growth of P. infestans.

Keywords

Phytophthora infestans V-8 Agar media Late Blight Ray Agar media
How to Cite This Article
Amira Al-Harethi, Qais Y. Abdullah, Hala J. Al-Jobory (2024). Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar. Sana'a University Journal of Applied Sciences and Technology, 103 - 106. DOI: https://doi.org/10.59628/jast.v2i1.768

Amira Al-Harethi, Qais Y. Abdullah, Hala J. Al-Jobory . “Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar.” Sana'a University Journal of Applied Sciences and Technology, 2024, pp. 103 - 106. https://doi.org/10.59628/jast.v2i1.768. Accessed 20 Sep. 2026.

Amira Al-Harethi, Qais Y. Abdullah, Hala J. Al-Jobory . 2024. “Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar.” Sana'a University Journal of Applied Sciences and Technology : 103 - 106. DOI: https://doi.org/10.59628/jast.v2i1.768

Amira Al-Harethi, Qais Y. Abdullah, Hala J. Al-Jobory (2024) ‘Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar’, Sana'a University Journal of Applied Sciences and Technology, pp. 103 - 106. doi: 10.59628/jast.v2i1.768

@article{jast2024_art768, author = {Amira Al-Harethi, Qais Y. Abdullah, Hala J. Al-Jobory }, title = {Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2024}, month = {February}, pages = {103 - 106}, doi = {10.59628/jast.v2i1.768}, url = {https://doi.org/10.59628/jast.v2i1.768},}

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Phytophthora infestans Culture Media: A Comparative Study of Cost-Effective V8 Agar and Rye Agar

Amira Al-Harethi Corresponding a.alharethi@su.edu.ye

Qais Y. Abdullah q.yusuf@su.edu.ye

Hala J. Al-Jobory h.aljobory@su.ede.ye

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Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen

Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Sara Mohammed Ahmed Mayas, Maysoon Abdulrahman Al Zubairy, Amira Ali Mohammed Al-Harethi, Sara Salem Saeed Baraba'a

1155-1163

2025-10-28

DOI: 10.59628/jast.v3i5.1643

Abstract

Background: Helicobacter pylori (H. pylori) is a bacterium frequently contracted during childhood and is the primary etiological agent of various gastrointestinal conditions, including gastritis, peptic ulcers, and gastric can- cer. The transmission of H. pylori predominantly occurs through person-to-person contact, most commonly via oral-oral or fecal-oral pathways. Its acquisition and persistence are strongly affected by various environmental and socioeconomic conditions. This study aimed to determine the prevalence of H. pylori infection and evaluate its association with multiple risk factors among individuals presenting with gastrointestinal disorders in Dhamar, Yemen. Methods: This cross-sectional study was conducted between January to December 2022. H. pylori infection was detected using the stool antigen test (HpSA), which qualitatively identifies H. pylori antigens in human fecal samples. Demographic and behavioral data including age, gender, smoking status, family size, number of rooms in the household, educational level, Khat (Catha edulis) consumption, and tobacco (Shamma) chewing were collected through structured interviews. Logistic regression models were used in the analysis of odds ratios (ORs) and their respective 95% confidence intervals (CIs), with a 5% significance level. Results: A total of 515 stool samples were collected from individuals in Dhamar Governorate, Yemen, to evaluate the prevalence of H. pylori infection. The overall infection rate was found to be 68.5%, corresponding to 353 positive cases. Among the 185 male participants, 107 (57.8%) tested positive, while 246 of the 330 female participants (74.5%) were positive. Although no significant association was found between infection rates and family size, statistically significant correlations were observed with several factors, including gender, age, number of rooms in the household, educational level, smoking status, khat chewing, and tobacco (Shamma) use. Conclusion: This study highlights the substantial prevalence of H. pylori infection in the Dhamar community. A comprehensive understanding of the associated risk factors is essential for devising effective strategies to control and ultimately eliminate the transmission of this pathogen within the Yemeni population.

Keywords

Helicobacter pylori Associated risk factors H.pylori antigen Yemen prevalence
How to Cite This Article
Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Sara Mohammed Ahmed Mayas, Maysoon Abdulrahman Al Zubairy, Amira Ali Mohammed Al-Harethi, Sara Salem Saeed Baraba'a (2025). Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen. Sana'a University Journal of Applied Sciences and Technology, 1155-1163. DOI: https://doi.org/10.59628/jast.v3i5.1643

Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Sara Mohammed Ahmed Mayas, Maysoon Abdulrahman Al Zubairy, Amira Ali Mohammed Al-Harethi, Sara Salem Saeed Baraba'a . “Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1155-1163. https://doi.org/10.59628/jast.v3i5.1643. Accessed 20 Sep. 2026.

Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Sara Mohammed Ahmed Mayas, Maysoon Abdulrahman Al Zubairy, Amira Ali Mohammed Al-Harethi, Sara Salem Saeed Baraba'a . 2025. “Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 1155-1163. DOI: https://doi.org/10.59628/jast.v3i5.1643

Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Sara Mohammed Ahmed Mayas, Maysoon Abdulrahman Al Zubairy, Amira Ali Mohammed Al-Harethi, Sara Salem Saeed Baraba'a (2025) ‘Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 1155-1163. doi: 10.59628/jast.v3i5.1643

@article{jast2025_art1643, author = {Salwa H. Alkhyat, Maged Ahmed Al-Garadi, Sara Mohammed Ahmed Mayas, Maysoon Abdulrahman Al Zubairy, Amira Ali Mohammed Al-Harethi, Sara Salem Saeed Baraba'a }, title = {Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {October}, pages = {1155-1163}, doi = {10.59628/jast.v3i5.1643}, url = {https://doi.org/10.59628/jast.v3i5.1643},}

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Prevalence and Risk Factors for Helicobacter pylori Infection among Gastrointestinal Patients in Dhamar City, Yemen

Salwa H. Alkhyat Corresponding s.alkhyat@su.edu.ye

Maged Ahmed Al-Garadi s.alkhyat@su.edu.ye

Sara Mohammed Ahmed Mayas s.alkhyat@su.edu.ye

Maysoon Abdulrahman Al Zubairy s.alkhyat@su.edu.ye

Amira Ali Mohammed Al-Harethi s.alkhyat@su.edu.ye

Sara Salem Saeed Baraba'a s.alkhyat@su.edu.ye

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the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen

W. A. alshuaibi, S. M. Alghalibi

303-308

2024-06-29

DOI: 10.59628/jast.v2i3.882

Abstract

Bacterial vaginal infection (BV) is an imbalance in the normal vaginal flora with decreased levels of the usual predominant lactobacilli. This study aimed to determine the prevalence of BV among women attending obstetrics and gynecology clinics in Sana’a city, Yemen; to isolate and identify the causative of VB; to assess risk factors associated with the prevalence of BV, and to determine the effect of some antibiotics. In this cross-sectional study, 150 vaginal swab samples were collected. All specimens were examined by microscope, cultural and biochemical tests, in addition to conducting an antibiotics sensitivity test for positive samples. In this study, BV was the highest among women aged less than 20 years old. The bacteria isolated were Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Enterobacter spp, Proteus mirabilis, Streptococcus pyogenes and Pseudomonas aeruginosa. The most effective antibiotics were Cefotaxime, Gentamycin and Ciprofloxacin.

Keywords

Bacterial vaginal infection Risk factors Antibiotics
How to Cite This Article
W. A. alshuaibi, S. M. Alghalibi (2024). the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen. Sana'a University Journal of Applied Sciences and Technology, 303-308. DOI: https://doi.org/10.59628/jast.v2i3.882

W. A. alshuaibi, S. M. Alghalibi . “the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2024, pp. 303-308. https://doi.org/10.59628/jast.v2i3.882. Accessed 20 Sep. 2026.

W. A. alshuaibi, S. M. Alghalibi . 2024. “the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 303-308. DOI: https://doi.org/10.59628/jast.v2i3.882

W. A. alshuaibi, S. M. Alghalibi (2024) ‘the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 303-308. doi: 10.59628/jast.v2i3.882

@article{jast2024_art882, author = {W. A. alshuaibi, S. M. Alghalibi }, title = {the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2024}, month = {June}, pages = {303-308}, doi = {10.59628/jast.v2i3.882}, url = {https://doi.org/10.59628/jast.v2i3.882},}

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the Prevalence of Bacterial Vaginal Infections among Women in Sana’a City, Yemen

W. A. alshuaibi Corresponding alshuaibi.w@su.edu.ye

S. M. Alghalibi s.alghalabi@su.edu.ye

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Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study

Kholood M. H. Ghlabe, Saad Al-Arnoot, Qais Y. M. Abdullah, Abdulrahman A. Humaid, Wadhah H Edrees, Ahlam M. H. Al-Matry

1259-1265

2025-12-28

DOI: 10.59628/jast.v3i6.2051

Abstract

Blood transfusion is a vital therapeutic procedure but carries the risk of transfusion-transmissible infections, particularly in resource limited settings. This cross-sectional study was conducted to determine the prevalence of infectious markers among voluntary male blood donors in Sana’a, Yemen, Brazil. A total of 200 donors aged 18–60 years were recruited between June 2024 and May 2025 from three major facilities: Al-Kuwait University Hospital, National Blood Transfusion and Research Center, and National Center for Central Public Health Labora-tories in Sana’a City, Yemen. Blood samples were screened for HIV, HBsAg, anti-HBc, HCV, syphilis (Treponema pallidum), and malaria using ELISA and microscopy. Overall, 25 donors (12.5%; 95% CI: 8.6–17.8) were positive for at least one infectious marker. Anti-HBc was the most common (7.0%), followed by malaria (5.5%), while HBsAg and HCV were less frequent (1.5% and 0.5%, respectively), and no cases of HIV or syphilis were detected. Statistical analysis showed significant associations between HCV and shamma use (p = 0.017) and between anti-HBc and educational level (p = 0.006), whereas smoking demonstrated a borderline association with anti-HBc (p = 0.057). No significant relationships were found between malaria or HBsAg and sociodemographic or behavioral variables. The results indicate that exposure to hepatitis B virus and malaria remains the predominant transfusion-related risk in this population, whereas exposure to HIV and syphilis appears negligible. These findings underscore the importance of strengthening donor education, sustaining HBV vaccination and screening programs, and improving malaria prevention strategies to enhance the safety of blood transfusion in Yemen

Keywords

Blood donors Infectious markers Transfusion-transmissible infections Hepatitis B virus Hepatitis C virus Sana’a Yemen
How to Cite This Article
Kholood M. H. Ghlabe, Saad Al-Arnoot, Qais Y. M. Abdullah, Abdulrahman A. Humaid, Wadhah H Edrees, Ahlam M. H. Al-Matry (2025). Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study. Sana'a University Journal of Applied Sciences and Technology, 1259-1265. DOI: https://doi.org/10.59628/jast.v3i6.2051

Kholood M. H. Ghlabe, Saad Al-Arnoot, Qais Y. M. Abdullah, Abdulrahman A. Humaid, Wadhah H Edrees, Ahlam M. H. Al-Matry . “Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1259-1265. https://doi.org/10.59628/jast.v3i6.2051. Accessed 20 Sep. 2026.

Kholood M. H. Ghlabe, Saad Al-Arnoot, Qais Y. M. Abdullah, Abdulrahman A. Humaid, Wadhah H Edrees, Ahlam M. H. Al-Matry . 2025. “Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study.” Sana'a University Journal of Applied Sciences and Technology : 1259-1265. DOI: https://doi.org/10.59628/jast.v3i6.2051

Kholood M. H. Ghlabe, Saad Al-Arnoot, Qais Y. M. Abdullah, Abdulrahman A. Humaid, Wadhah H Edrees, Ahlam M. H. Al-Matry (2025) ‘Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study’, Sana'a University Journal of Applied Sciences and Technology, pp. 1259-1265. doi: 10.59628/jast.v3i6.2051

@article{jast2025_art2051, author = {Kholood M. H. Ghlabe, Saad Al-Arnoot, Qais Y. M. Abdullah, Abdulrahman A. Humaid, Wadhah H Edrees, Ahlam M. H. Al-Matry }, title = {Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {December}, pages = {1259-1265}, doi = {10.59628/jast.v3i6.2051}, url = {https://doi.org/10.59628/jast.v3i6.2051},}

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Prevalence of Infectious Markers among Blood Donors in Sana’a, Yemen: A Cross-Sectional Study

Kholood M. H. Ghlabe kh.almatry@su.edu.ye

Saad Al-Arnoot Corresponding s.alarnoot@su.edu.ye

Qais Y. M. Abdullah q.yusuf@su.edu.ye

Abdulrahman A. Humaid a-hamid@su.edu.ye

Wadhah H Edrees edress2020@gmail.com

Ahlam M. H. Al-Matry ahlam.micro@gmail.com

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Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen

Abdulraqeb Alshami, Saleh A. M. A. ALomaisi, Abdu-Alraoof Al-shawkany, Hamid Ali Alrefaiey, Aziz Sharaf Al azazi, Muhammad Dhifallah Tahir, Faisal Al-sharabi

492-498

2024-12-31

DOI: 10.59628/jast.v2i6.1150

Abstract

Background: Colic is a major health problem in controlled horse populations globally. It is a multifactorial and complex disorder. In Yemen, the prevalence, risk factors, and results of horse colic are unclear.

Objectives: The current study aimed to determine the prevalence, risk factors, and haemato-biochemical changes associated with colic in horses in Sana’a, Yemen.

Methods: A prospective study was conducted from September 2023 to February 2024. A total of 254 horses were examined under field conditions. Colic was identified based on physical examination, clinical symptoms, and rectal examination. A questionnaire was distributed to the horse owners to assess the risk factors while the clinical examination was performed, and blood samples were obtained to estimate the haemato biochemical parameters in horses with colic.

Results: out of a total of 254 horses, the overall prevalence of colic in horses was found to be 11.81% (30 cases) and of these, 73.3% had spasmodic colic whereas 26.7% had impaction. The Means of respiration rate, pulse rate, and capillary refill time were significantly increased in horses affected with colic than healthy control animals (P<0.05). The horses with colic showed a significant association between cases with age (p<0.098), gender (p<0.023), deworming frequency (p<0.001), and previous exposure to colic (p<0.001). In hematological indices, the mean packed cell volume in horses with colic was found to be dramatically increased (P<0.0001), while the means of total platelet count, lymphocyte count, and neutrophil count were found to be significantly decreased (P<0.001). Serum biochemical indices, including aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, creatinine, glucose, albumin, total protein, and alkaline phosphate, were significantly higher in horses with colic compared to healthy controls (P<0.0001).

Conclusions: This is the first study that documented the prevalence of colic in horses and identified risk factors and haemato-biochemical changes associated with colic in Sana’a province, Yemen. This information is a crucial first step in understanding the impact of colic in horses and developing ways to reduce the risk of colic.

Keywords

Horses Colic Sana’a Prevalence Risk factors Hematological Biochemical
How to Cite This Article
Abdulraqeb Alshami, Saleh A. M. A. ALomaisi, Abdu-Alraoof Al-shawkany, Hamid Ali Alrefaiey, Aziz Sharaf Al azazi, Muhammad Dhifallah Tahir, Faisal Al-sharabi (2024). Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen. Sana'a University Journal of Applied Sciences and Technology, 492-498. DOI: https://doi.org/10.59628/jast.v2i6.1150

Abdulraqeb Alshami, Saleh A. M. A. ALomaisi, Abdu-Alraoof Al-shawkany, Hamid Ali Alrefaiey, Aziz Sharaf Al azazi, Muhammad Dhifallah Tahir, Faisal Al-sharabi . “Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2024, pp. 492-498. https://doi.org/10.59628/jast.v2i6.1150. Accessed 20 Sep. 2026.

Abdulraqeb Alshami, Saleh A. M. A. ALomaisi, Abdu-Alraoof Al-shawkany, Hamid Ali Alrefaiey, Aziz Sharaf Al azazi, Muhammad Dhifallah Tahir, Faisal Al-sharabi . 2024. “Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 492-498. DOI: https://doi.org/10.59628/jast.v2i6.1150

Abdulraqeb Alshami, Saleh A. M. A. ALomaisi, Abdu-Alraoof Al-shawkany, Hamid Ali Alrefaiey, Aziz Sharaf Al azazi, Muhammad Dhifallah Tahir, Faisal Al-sharabi (2024) ‘Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 492-498. doi: 10.59628/jast.v2i6.1150

@article{jast2024_art1150, author = {Abdulraqeb Alshami, Saleh A. M. A. ALomaisi, Abdu-Alraoof Al-shawkany, Hamid Ali Alrefaiey, Aziz Sharaf Al azazi, Muhammad Dhifallah Tahir, Faisal Al-sharabi }, title = {Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2024}, month = {December}, pages = {492-498}, doi = {10.59628/jast.v2i6.1150}, url = {https://doi.org/10.59628/jast.v2i6.1150},}

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Prevalence, Risk Factors, and Haemato-Biochemical Changes Associated with Colic in Horses in Sana’a Province, Yemen

Abdulraqeb Alshami Corresponding a.shami@su.edu.ye

Saleh A. M. A. ALomaisi a.shami@su.edu.ye

Abdu-Alraoof Al-shawkany a.shami@su.edu.ye

Hamid Ali Alrefaiey a.shami@su.edu.ye

Aziz Sharaf Al azazi a.shami@su.edu.ye

Muhammad Dhifallah Tahir a.shami@su.edu.ye

Faisal Al-sharabi a.shami@su.edu.ye

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Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen

Hadeel K.M. Khalifa, Hala J. Al Jobory, Saeed M. Al Ghalibi

1378-1386

2025-12-28

DOI: 10.59628/jast.v3i6.2113

Abstract

Public cemeteries are potential sources of contamination by groundwater pathogens. Many settlements in Yemen are located near cemeteries, and rely on groundwater for consumption. However, no information is available on the effect of cemeteries on groundwater quality in Yemen. Therefore, this study, which is the first of its kind in Yemen, aimed to assess the bacteriological and physiochemical quality of groundwater from four wells in proximity to the Al Amody cemetery in Dhamar city, Yemen, with a focus on evaluating potential contamination risks to local communities relying on untreated well water. Water samples were analyzed for total aerobic heterotrophic bacteria (HPC), total coliforms, E. coli, fecal indicators, and a range of pathogenic bacteria, along with physicochemical parameters, including pH, temperature, total dissolved solids (TDS), and electrical conductivity (EC). Results revealed severe microbial contamination in wells closest to the cemetery, particularly Well 1 (5 m from the site), where HPC and multiple bacterial indicators—including total coliforms, fecal coliforms, E. coli , Salmonella spp., Vibrio cholerae , Proteus spp., and Pseudomonas aeruginosa —were present at "too numer- ous to count" (TNTC) levels, far exceeding WHO and Yemen standards. Wells 2 (147 m distance) and 3 (323 m distance) showed moderate contamination, with E. coli detected at 55 CFU/mL and 17 CFU/mL, respectively, whereas all bacterial groups were absent in the control well (Well 4, 1,169 m away). Physicochemical analysis indicated TDS and EC levels below the Yemen standard limit (1500 mg/L), although two wells exceeded the WHO guideline (1000 mg/L); pH (7.6–8.3) and temperature (20–28 Â °C) remained within acceptable ranges. These findings confirm that groundwater near cemeteries in areas with permeable soils and high water tables is highly vulnerable to fecal and pathogenic contamination, posing significant public health risks.

Keywords

Groundwater contamination Cemetery leachate Fecal coliforms E. coli Waterborne pathogens
How to Cite This Article
Hadeel K.M. Khalifa, Hala J. Al Jobory, Saeed M. Al Ghalibi (2025). Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen. Sana'a University Journal of Applied Sciences and Technology, 1378-1386. DOI: https://doi.org/10.59628/jast.v3i6.2113

Hadeel K.M. Khalifa, Hala J. Al Jobory, Saeed M. Al Ghalibi . “Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen.” Sana'a University Journal of Applied Sciences and Technology, 2025, pp. 1378-1386. https://doi.org/10.59628/jast.v3i6.2113. Accessed 20 Sep. 2026.

Hadeel K.M. Khalifa, Hala J. Al Jobory, Saeed M. Al Ghalibi . 2025. “Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen.” Sana'a University Journal of Applied Sciences and Technology : 1378-1386. DOI: https://doi.org/10.59628/jast.v3i6.2113

Hadeel K.M. Khalifa, Hala J. Al Jobory, Saeed M. Al Ghalibi (2025) ‘Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen’, Sana'a University Journal of Applied Sciences and Technology, pp. 1378-1386. doi: 10.59628/jast.v3i6.2113

@article{jast2025_art2113, author = {Hadeel K.M. Khalifa, Hala J. Al Jobory, Saeed M. Al Ghalibi }, title = {Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen}, journal = {Sana'a University Journal of Applied Sciences and Technology}, year = {2025}, month = {December}, pages = {1378-1386}, doi = {10.59628/jast.v3i6.2113}, url = {https://doi.org/10.59628/jast.v3i6.2113},}

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Proximity Matters: Spatial Gradient of Bacterial Pollution in Groundwater in the Vicinity of Al Amodi Cemetery in Dhamar City, Yemen

Hadeel K.M. Khalifa h.aljubouri@su.edu.ye

Hala J. Al Jobory Corresponding h.aljubouri@su.edu.ye

Saeed M. Al Ghalibi h.aljubouri@su.edu.ye

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