Evaluation of Antioxidant and Antibacterial Activities of Leaf and Seeds Extracts of Neem (Azadirachta indica) Using Different Extraction
Keywords:
Azadirachta indica, Soxhlet ethanolic seeds extract , Maceration aqueous leaf extract, Mac- eration ethanolic leaf extract, Antioxidant , Antibacterial activityAbstract
Neem (Azadirachta indica), has long been recognized for its medicinal properties. This study evaluates the antibacterial, antioxidant using the Ferric-Biperidine Reducing Capacity of Total Antioxidants (FBRC), Ferric-Reduced Antioxidant Method (FRAP), and scavenging activity by the 1, 1-diphenyl 2-picrylhyorazyl (DPPH) method. The total phenolic content of neem leaf and seed extracts was determined using Folin-Ciocalteu Aliquot method. Among the extracts analyzed, the aqueous soaked leaf extract exhibited the highest antioxidant activity, followed by the ethanolic-soaked leaf extracts, while the ethanolic Soxhlet seed extracts demonstrated the lowest activity. the total phenolic content was the highest in ethanolic Soxhlet seed extracts (6.319 mg/mg AAE) and aqueous soaked leaf extracts (4.838 mg/mg), with ethanolic soaked leaf extracts showing the lowest concentration (4.165 mg/mg). Antibacterial activity against Escherichia coli and Staphylococcus aureus was observed exclusively in ethanol-soaked leaf extracts, with significant inhibition of S. aureus (18 mm). These findings high-light the potent antioxidant and antimicrobial properties of neem extracts, supporting their potential application in medicinal treatments.
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Copyright (c) 2024 Maher A. Almaqtari, Faten H. Thamer, Adlia A. Abduljabbar, Amani A. AlGaadbi, Afraah M. Alfaatesh, Hussein M. A. Al-Maydama
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