Isolation and Identification of Pathogenic Bacteria from Patients with Dental Plaque in Sana’a City, Yemen

Abstract

Oral flora is important for maintaining dental health, but some situations may disturb the oral microbiota, which can support the growth of Microbial Pathogens. 28 pathogenic bacteria:17 G+ve and 11 G-ve) were isolated from dental plaques of 192 dental patients and identified by the VITEK 2 Compact System after culturing and studying their morphological features using Gram stain. About 13 (9 G+vecocci and 4 G-ve bacilli) and 24 (13 G+ve cocci, 1 G+ve bacilli, and 10 G-ve bacilli) pathogenic bacteria were isolated from the dental plaques of 46 males and 146 Female patients, respectively. Moreover, Streptococcus mitis exhibited the highest prevalence among the 28 isolated pathogenic bacteria. Furthermore, Serratia marcescens, and Streptococcus parasanguinis showed the highest mean number of colonies among the pathogenic bacteria isolated from male patients with dental plaques. Similarly, Serratia odorifera exhibited the highest mean number of colonies among the pathogenic bacteria isolated from female Patients with dental plaques.